J Neuroimmunol 2002 Sep;130(1-2):233
Muraro P, Kalbus M, Afshar G, McFarland H, Martin R.
Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, Bldg. 10, Room 5B-16, National Institutes of Health, 10 Center Drive MSC1400, 20892, Bethesda, MD, USA
T cell responses targeting myelin antigens are possibly involved in the pathogenesis of demyelinating diseases, such as multiple sclerosis (MS).
Little is known about human T cell responses to 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), the third most abundant myelin protein.
We examined the primary peripheral T cell response to CNPase and characterized CNPase-specific CD4+ long-term T cell lines (TCL) from MS patients and healthy donors.
The strongest primary responses were found in two MS patients with very active disease and were directed against CNP(343-373).
We identified immunodominant epitope clusters in the regions CNP(343-373) and (356-388) that were recognized in the context of MS-associated HLA-DR2 and DR4 molecules.
These data provide the immunological basis for further investigation of CNPase as a potential target self-antigen in MS.