Neurosci Lett. 2003 Dec 4;352(2):101-4
Gomes AC, Morris M, Stawiarz L, Jonsson G, Putheti P, Bronge L, Link H, Hillert J.
Division of Neurology, Neurotec Department, Karolinska Institute, Huddinge University Hospital R54, SE-141 86, Stockholm, Sweden
Magnetic resonance imaging (MRI) allows examining inflammation and central nervous system (CNS) tissue damage in patients suffering from multiple sclerosis (MS), a demyelinating disease of the CNS.
Using real-time PCR, we quantified mRNA levels of apoptosis regulators CD95, CD95 ligand, caspase-8, -10 and cellular FLICE-inhibitory protein (cFLIP), and cytokines IL-10 and TNF-alpha in blood mononuclear cells of MS patients at the time of MRI examination.
Patients with detectable gadolinium-enhancing lesions had lower expression of CD95 and caspase-8 (P<0.05).
Lesion load and brain atrophy did not correlate with expression levels of any of the target molecules studied.
Disease duration correlated positively with both FLIP/caspase-8 and CD95/CD3 ratios (P<0.05).
These results support the notion that the CD95-dependent pathway plays a complex role in the regulation of survival of activated immune cells in MS.