Journal of Neuroimmunology, Vol. 133 (1-2) (2002) pp. 72-80
Susanne A. Wolf a, Jasmin Fisher b, Ingo Bechmann a, Barbara Steiner a, Erik Kwidzinski a and Robert Nitsch a
a Department of Cell and Neurobiology, Institute of Anatomy, Humboldt-University Hospital Charitè, 10098 Berlin, Germany
b Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
This study analyzes how the antigen specificity, the subtype, and the activation state of T cells modulate their recently discovered neuroprotective potential.
We assessed the prevention from neuronal damage in organotypic entorhinal-hippocampal slice cultures after co-culture with Th1 and Th2 cells either specific for myelin basic protein (MBP) or ovalbumin (OVA).
We found that MBP-specific Th2 cells were the most effective in preventing central nervous system (CNS) tissue from secondary injury.
This neuroprotective T cell effect appears to be mediated by soluble factors.
After stimulation with phorbol myristate acetate and ionomycin, all T cells were most effective in preventing neuronal death.
Our data show that the T cell subtype and activation state are important features in determining the neuroprotective potential of these cells.
© 2002 Elsevier Science B.V.