J Neuroimmunol 2003 May;138(1-2):115-22
Malamud V, Vaaknin A, Abramsky O, Mor M, Burgess LE, Ben-Yehudah A, Lorberboum-Galski H.
Department of Cellular Biochemistry and Human Genetics, Hebrew University, Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel
Presence of mast cells and an increase in the concentration of their products has been reported in multiple sclerosis (MS) plaques.
The most abundant secretory mediator of the human mast cell is the tetrameric protease tryptase.
We demonstrate that tryptase can activate peripheral mononuclear cells (PBMCs), isolated from healthy donors as well as MS patients for the release of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6 and IL-1beta.
Cytokine secretion was significantly higher in secondary progressive (SP) MS patients and healthy control (HC) individuals than in relapsing-remitting (RR) patients.
Our findings suggest that tryptase is, most probably, an important mediator of inflammation in MS.