Neurosci Lett 2003 Apr 3;340(1):5-8
Lombardi G, Miglio G, Canonico PL, Naldi P, Comi C, Monaco F.
DISCAFF Department, University of Piemonte Orientale "Amedeo Avogadro", Via Bovio, 6, 28100, Novara, Italy
Multiple sclerosis (MS) is an immune-mediate, inflammatory and demyelinating disease of the central nervous system (CNS).
Since glutamate (Glu) is a modulator of T lymphocyte function and Glu excitotoxicity has been proposed as one of the mechanisms of the demyelination, we studied the responses of T lymphocytes from normal controls (NC), MS or other non-inflammatory neurological disease (ONND) patients to Glu, by measuring phytohemagglutinin-induced intracellular Ca(2+) ([Ca(2+)](i)) rise (Fura-2 method) and cell proliferation (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay).
No differences in the Glu (1 &mgr;M)-induced potentiation of the [Ca(2+)](i) rise were measured in T lymphocytes from all groups of subjects, while a significant decrease in the Glu (1 mM)-induced inhibition of cell proliferation was observed in T lymphocytes from MS patients.
These data demonstrate that MS T lymphocytes abnormally respond to Glu.