J Neuroimmunol 2002 Feb;123(1-2):193-8
Dyment DA, Steckley JL, Willer CJ, Armstrong H, Sadovnick AD, Risch N, Ebers GC.
The Wellcome Trust Center for Human Genetics, University of Oxford, Oxford, UK
Two polymorphisms of the CTLA-4 gene were genotyped in 232 sibling pairs affected with multiple sclerosis (MS) from 185 families.
The CTLA-4 polymorphisms genotyped were a 3prime prime or minute untranslated (AT)(n) microsatellite and an alanine/threonine RFLP of exon 1.
There was no evidence observed for linkage by either identity-by-descent (ibd) or identity-by-state (ibs) methods.
A transmission disequilibrium test (TDT) was performed and no preferential transmission of alleles was observed.
Upon stratification of patients, there was no preferential transmission observed based upon gender, by presence or absence of HLA*DRB1*15, by ethnicity or by clinical course of the disease.
CTLA-4 does not appear to be a major MS susceptibility locus in Canadian multiplex families.