J Neurol Sci. 2003 Aug 15;212(1-2):21-30
't Hart BA, Vogels JT, Spijksma G, Brok HP, Polman C, van der Greef J.
Department of Immunobiology, Biomedical Primate Research Centre, P.O. Box 3306, 2280 GH, Rijswijk, The Netherlands
Proton nuclear magnetic resonance (1H-NMR) spectroscopy in combination with pattern recognition techniques were used to investigate the composition of organic compounds in urines from patients with multiple sclerosis (MS), patients with other neurological diseases (OND) and healthy controls (H).
Using a valid animal model of MS, namely the common marmoset (Callithrix jacchus) model of experimental autoimmune encephalomyelitis (EAE), the relation of disease progression and alteration of the urine composition was investigated.
Urine samples were collected during different stages of EAE, either induced with whole human myelin or with the myelin protein MOG in complete adjuvant.
The urine samples were analysed with 1H-NMR spectroscopy allowing simultaneous detection of an array of compounds.
Spectral differences between urines from EAE-affected and healthy monkeys were assessed with multivariate analysis.
Evidence is provided that development of EAE is associated with changes in the chemical composition of the urine, in particular of compounds with NMR peaks in the region of the spectrum between 0.5 and 3.50 ppm.
In addition, we found preliminary evidence for differences between urines from MS, OND and H groups.