More MS news articles for June 2002

Genes Next To One Another May Be Expressed Together

http://unisci.com/stories/20022/0618022.htm

18-Jun-2002

Our current understanding of gene expression, the fundamental process by which proteins are made from the instructions encoded in DNA, is that the process is tightly controlled so that the correct amount of each protein is produced in the right place at the right time.

But new research by Paul Spellman and Gerald Rubin of Howard Hughes Medical Institute and University of California Berkeley indicates that some groups of around 15 genes that just happen to be located next to each other on chromosomes are instead routinely expressed together.

The research is featured in the inaugural issue of Journal of Biology.

Genes within a block are not related to one another by structure or function: they are simply neighbors within the genome. These conclusions were drawn from experiments using DNA-coated microarrays to monitor the expression of all the genes in the genome of the fruitfly.

Microarray experiments allow researchers to look at the expression of a large number of genes in a single experiment and ask which genes are being turned on (or expressed) in a particular cell type, at a certain time, under defined conditions.

Spellman and Rubin analyzed gene expression data from 88 independent microarray experiments using DNA extracted from the fruitfly, Drosophila
melanogaster.

Then, instead of the usual practice of searching the microarray data for groups of genes that showed similar expression profiles, they arranged the expression data in the order in which the genes occur along the fly’s chromosomes.

To their surprise, Spellman and Rubin found over 200 large groups of adjacent and similarly expressed genes. Each of these groups contained between 10 and 30 genes, and together the groups accounted for approximately 20% of the genes in the fly’s genome.

This suggests that there is a previously unsuspected mechanism of regulating gene expression, which works on blocks of genes depending on their position along a chromosome.

Having ruled out other possible explanations, Spellman and Rubin speculate that the expression of gene groups might prove to reflect some feature of the chromatin -- the packaging of DNA and proteins into chromosomes -- that, in allowing an "important" gene to be expressed, also leads to the expression of neighboring genes, more by accident than by design.

This somewhat "sloppy" regulation of gene expression may be used when it is relatively unimportant how much of each protein is produced, they suggest, whereas precise control of the gene is used when the level of a protein is critical.

Only time will tell if the expression of groups of genes located next to each other is a universal feature of higher organisms, but Spellman and Rubin speculate that "as further experiments are carried out it may be found that our observation will grow to include all genes".

This article is available free of charge on the date of publication, (today), in line with the publisher's policy of open access to original research at Journal of Biology.

The inaugural issue of Journal of Biology also contains a research news article describing the findings and the background to them, by science writer Jonathan Weitzman, and a commentary by Professor Peter Suber of Earlham College, on the importance of open access to research.

Journal of Biology's Editor in Chief is Professor Martin Raff of University College London (UCL) in the UK. Its publisher is BioMed Central.
 

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