More MS news articles for July 2002

Imaging inflammation: Direct visualization of perivascular cuffing in EAE by magnetic resonance microscopy

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12112500&dopt=Abstract

J Magn Reson Imaging 2002 Jul;16(1):28-36
Gareau PJ, Wymore AC, Cofer GP, Johnson GA.
The John P. Robarts Research Institute, London, Ontario, Canada.

Purpose

To determine if the architectural features revealed by magnetic resonance microscopy (MRM) allow one to detect microscopic abnormalities associated with neuroinflammation in fixed brain sections from animals with experimental allergic encephalomyelitis (EAE), an animal model for multiple sclerosis (MS).Materials and

Methods

Imaging was performed at the Center for In Vivo Microscopy (CIVM) using a 9.4-Tesla, 89-mm bore, superconducting magnet with actively shielded gradients capable of 850 mT/m. A number of MR contrasts and spatial resolutions were explored.

Results

The assessment of EAE brain showed that it is possible to visualize perivascular cuffing in vitro by MRM on three-dimensional T1 proton stains.

Conclusion

Inflammatory cell infiltration is a prerequisite for the development of lesions in EAE and MS. Thus, the ability to directly detect individual perivascular cuffs of inflammation may provide a useful means of monitoring the time course of inflammatory events, as conventional histopathological scoring of perivascular cuffs is utilized, but in the absence of sectioning and staining.

Copyright 2002 Wiley-Liss, Inc