J Neuroimmunol 2001 Jun 1;116(2):213-9
Ramanathan M, Weinstock-Guttman B,
Nguyen LT, Badgett D, Miller C, Patrick K, Brownscheidle C, Jacobs L
Objectives:
To use DNA arrays to identify differences
in gene expression associated with relapsing-remitting (RR) MS.
Methods:
Total RNA was isolated from monocyte
depleted peripheral blood mononuclear cells of 15 RR MS patients and 15
age- and sex-matched controls. The RNA was reverse transcribed to radiolabeled
cDNA and the resultant cDNA was used to probe a DNA array containing over
4000 named human genes. The binding of radiolabeled cDNA to the probes
on the array was measured by phosphorimager.
Results:
Of more than 4000 genes tested, only
34 were significantly different in RR-MS patients from controls. Of these,
25 were significantly increased and 9 significantly decreased in the RR
MS patients. Twelve of these genes have inflammatory and/or immunological
functions that could be relevant to the MS disease process. The potentially
relevant genes that were elevated (15% to 28%) were P protein, LCK, cAMP
responsive element modulator, IL-7 receptor, matrix metalloproteinase-19,
M130 antigen, and peptidyl-prolyl isomerase. Those that were significantly
decreased (15% to 35%) were SAS transmembrane 4 superfamily protein, STRL22
(C-C chemokine receptor 6), AFX protein, DNA fragmentation factor-45 and
immunoglobulin gamma 3 (Gm marker).
Conclusions:
The RR-MS disease effect was relatively
restricted and most of the mRNAs tested were not different from the normal
controls. However, there were significant differences identified in the
expression of a subset of mRNAs, including 13 with inflammatory/immune
functions that could be relevant to MS. The systematic use of DNA arrays
can provide insight into the dynamic cellular pathways involved in MS pathogenesis
and its phenotypic heterogeneity.
PMID: 11438176, UI: 21331903
Department of Pharmaceutics, 543
Cooke Hall, State University of New York at Buffalo, 14260-1200, Buffalo,
NY, USA