More MS news articles for Jan 2002

Detection of oligoclonal immunoglobulins in cerebrospinal fluid by an immunofixation-peroxidase method

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11751552&dopt=Abstract

Clin Chem 2002 Jan;48(1):167-73
Richard S, Miossec V, Moreau JF, Taupin JL.
Laboratory of Immunology, Centre Hospitalier Regional et Universitaire de Bordeaux, 33076 Bordeaux, France.

BACKGROUND:

The detection of intrathecal synthesis of immunoglobulins is used in the diagnosis of multiple sclerosis (MS). We tested the semiautomated immunofixation-peroxidase (IFPOD) technique, which uses high-resolution agarose gel electrophoresis (HRAGE) directly followed by immunofixation with a peroxidase-labeled anti-IgG antiserum to detect oligoclonal immunoglobulins in cerebrospinal fluid (CSF).

METHODS:

We analyzed 230 consecutive matched serum/CSF pairs that arrived in the laboratory over a 6-month period with both IFPOD and our routine techniques, immunofixation electrophoresis (IFE) and HRAGE. For IFPOD, CSF samples were not concentrated before testing.

RESULTS:

Among the 230 samples were 12 clinically definite MS, 33 clinically probable, and 20 clinically possible MS samples. IFPOD and HRAGE + IFE each detected oligoclonal IgG in CSF in 10, 16, and 7 cases of these respective groups. For clinically definite MS, sensitivity and specificity (95% confidence intervals) were, respectively, 83% (51-97%) and 79% (73-84%).

CONCLUSIONS:

The IFPOD technique performs comparably to other analytical methods, without the requirement for sample concentration, and may represent an attractive alternative in testing for intrathecal immunoglobulin synthesis.
 

PMID: 11751552 [PubMed - in process]