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More MS news articles for February 2003

TNF-alpha and -beta gene polymorphisms in multiple sclerosis: a highly significant role for determinants in the first intron of the TNF-beta gene

Autoimmunity 2002 Sep;35(6):377-80
Fernandes Filho JA, Vedeler CA, Myhr KM, Nyland H, Pandey JP.
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA.

Tumor necrosis factor (TNF)-alpha and TNF-beta are proinflammatory cytokines that have been implicated in the pathogenesis of several autoimmune diseases.

The aim of this investigation was to determine whether a determinant in the first intron of the TNF-beta gene (TNF-beta(+252)) and two promoter-region polymorphisms of the TNF-alpha gene (TNF-alpha(-308) and TNF-alpha(-238)) affect susceptibility to multiple sclerosis (MS).

DNA samples from 133 Caucasian MS patients and 148 healthy controls from Norway were genotyped for several polymorphic determinants, using polymerase chain reaction-based restriction fragment length polymorphisms (PCR-RFLP) methods.

TNF-beta(+252) genotypes were significantly associated with MS: The frequency of TNF-beta 2,2 was increased (p = 0.00009) while the frequency of TNF-beta 1,2 was decreased (p = 0.0012) in MS patients as compared to controls.

TNF-alpha genotypes were not associated with MS.

These results suggest that the TNF-beta gene plays a significant role in the etiopathogenesis of MS.