J Neuroimmunol. 2004 Jan;146(1-2):126-132
Haerter K, Vroon A, Kavelaars A, Heijnen CJ, Limmroth V, Espinosa E, Schedlowski M, Elsenbruch S.
Department of Medical Psychology, University of Essen Medical School, Hufelandstr. 55, 45122, Essen, Germany
To analyze the effects in vitro of alpha- and beta-adrenoceptor agonists on splenocyte proliferation and on proinflammatory cytokine production in splenocytes and peritoneal macrophages (MF) in different stages of EAE.
Splenocytes and peritoneal macrophages were harvested in the acute phase of EAE and in remission, and from controls.
The beta-agonist terbutaline, the alpha(1)-agonist methoxamine, and the alpha(2)-agonist UK-14304 were added with ConA or lipopolysaccharide (LPS).
TNF-alpha and IFN-gamma contents in supernatant and splenocyte proliferation were determined.
Terbutaline and UK-14304 significantly suppressed TNF-alpha production by MF.
However, EAE acute phase rats were resistant to the suppressive effect of UK-14304.
Terbutaline significantly suppressed IFN-gamma and TNF-alpha production by splenocytes.
EAE acute phase and remission animals showed reduced terbutaline-induced inhibition of IFN-gamma production.
Disturbed sympathetic-immune communication in EAE is characterized by alterations in adrenergic sensitivity via both alpha- and beta-adrenergic pathways.