J Neuroimmunol. 2004 May;150(1-2):163-77
Iglesias AH, Camelo S, Hwang D, Villanueva R, Stephanopoulos G, Dangond F.
Laboratory of Transcriptional and Immune Regulation, Brigham and Women's Hospital, Department of Neurology, Harvard Medical School, Boston, MA, USA.
We performed microarray analysis of peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients and detected a profile of immune cell activation, autoantigen upregulation, and enhanced E2F pathway transcription.
Accordingly, E2f1-deficient mice manifested only mild disability upon induction of experimental autoimmune encephalomyelitis (EAE).
Furthermore, PBMCs from Avonex-treated patients had lower expression of E2F targets.
The profile was enriched in genes known to harbor MS-associated polymorphisms, or localized to MS susceptibility chromosomal regions.
Our study shows that PBMC microarrays reflect MS pathobiology that can be validated in the EAE model.